Cluster 3k PBMCs with Seurat - Workflow - SCTransform Version

Run Workflow in Galaxy

• Galaxy Main
• Galaxy Australia
• Galaxy Europe
• Galaxy France
• Import to another server (≥23.0+ only!)

flowchart TD
  0["ℹ️ Input Dataset\nmatrix.mtx"];
  style 0 stroke:#2c3143,stroke-width:4px;
  1["ℹ️ Input Dataset\ngenes.tsv"];
  style 1 stroke:#2c3143,stroke-width:4px;
  2["ℹ️ Input Dataset\nbarcodes.tsv"];
  style 2 stroke:#2c3143,stroke-width:4px;
  3["ℹ️ Input Dataset\nCanonical Markers"];
  style 3 stroke:#2c3143,stroke-width:4px;
  4["Input 3k PBMCs"];
  2 -->|output| 4;
  1 -->|output| 4;
  0 -->|output| 4;
  11e22da6-4b95-43a4-ab8c-63567fcca50c["Output\nInput 3k PBMC"];
  4 --> 11e22da6-4b95-43a4-ab8c-63567fcca50c;
  style 11e22da6-4b95-43a4-ab8c-63567fcca50c stroke:#2c3143,stroke-width:4px;
  5["Seurat Data Management"];
  4 -->|rds_out| 5;
  6["Mitochondrial Annotations"];
  4 -->|rds_out| 6;
  7["Seurat Visualize"];
  6 -->|rds_out| 7;
  8["Seurat Visualize"];
  6 -->|rds_out| 8;
  9["Seurat Visualize"];
  6 -->|rds_out| 9;
  10["Filtered Dataset"];
  6 -->|rds_out| 10;
  11["Preprocessed Data"];
  10 -->|rds_out| 11;
  112976dd-410e-46be-b063-83e631882b4f["Output\nPreprocessed Data"];
  11 --> 112976dd-410e-46be-b063-83e631882b4f;
  style 112976dd-410e-46be-b063-83e631882b4f stroke:#2c3143,stroke-width:4px;
  12["Seurat Data Management"];
  11 -->|rds_out| 12;
  13["Seurat Visualize"];
  11 -->|rds_out| 13;
  14["PCA Results"];
  11 -->|rds_out| 14;
  15["Seurat Visualize"];
  14 -->|rds_out| 15;
  16["Seurat Visualize"];
  14 -->|rds_out| 16;
  17["Seurat Visualize"];
  14 -->|rds_out| 17;
  18["Seurat Visualize"];
  14 -->|rds_out| 18;
  19["Seurat Visualize"];
  14 -->|rds_out| 19;
  20["Seurat Visualize"];
  14 -->|rds_out| 20;
  21["Seurat Visualize"];
  14 -->|rds_out| 21;
  22["Seurat Find Clusters"];
  14 -->|rds_out| 22;
  23["Seurat Find Clusters"];
  22 -->|rds_out| 23;
  24["UMAP Results"];
  23 -->|rds_out| 24;
  25["Seurat Visualize"];
  24 -->|rds_out| 25;
  26["Seurat Visualize"];
  24 -->|rds_out| 26;
  27["Seurat Visualize"];
  24 -->|rds_out| 27;
  28["DE Markers"];
  24 -->|rds_out| 28;
  29["Seurat Visualize"];
  24 -->|rds_out| 29;
  30["Seurat Visualize"];
  24 -->|rds_out| 30;
  31["Seurat Visualize"];
  24 -->|rds_out| 31;
  32["Seurat Visualize"];
  24 -->|rds_out| 32;
  33["Annotated Clusters"];
  24 -->|rds_out| 33;
  f009afd0-52ea-40c7-b603-861b79a562f3["Output\nAnnotated Clusters"];
  33 --> f009afd0-52ea-40c7-b603-861b79a562f3;
  style f009afd0-52ea-40c7-b603-861b79a562f3 stroke:#2c3143,stroke-width:4px;
  34["DE Markers Tabular"];
  28 -->|markers_tabular| 34;
  35["Seurat Visualize"];
  33 -->|rds_out| 35;
  36["Seurat Visualize"];
  3 -->|output| 36;
  33 -->|rds_out| 36;
  37["Input DE Markers"];
  34 -->|tabular| 37;
  a6eb57cd-e2cb-4f52-b211-b48fcf4cad06["Output\nInput DE Markers"];
  37 --> a6eb57cd-e2cb-4f52-b211-b48fcf4cad06;
  style a6eb57cd-e2cb-4f52-b211-b48fcf4cad06 stroke:#2c3143,stroke-width:4px;
  38["Seurat Visualize"];
  37 -->|out_file1| 38;
  33 -->|rds_out| 38;

Inputs

Outputs

Tools

To use these workflows in Galaxy you can either click the links to download the workflows, or you can right-click and copy the link to the workflow which can be used in the Galaxy form to import workflows.

Importing into Galaxy

Hands-on: Importing a workflow

• Click on Workflow on the top menu bar of Galaxy. You will see a list of all your workflows.
• Click on galaxy-upload Import at the top-right of the screen
• Provide your workflow
  • Option 1: Paste the URL of the workflow into the box labelled “Archived Workflow URL”
  • Option 2: Upload the workflow file in the box labelled “Archived Workflow File”
• Click the Import workflow button

Below is a short video demonstrating how to import a workflow from GitHub using this procedure:

Version History

For Admins

Installing the workflow tools

wget https://training.galaxyproject.org/training-material/topics/single-cell/tutorials/scrna-seurat-pbmc3k/workflows/Seurat_PBMC_Workflow_SCT.ga -O workflow.ga
workflow-to-tools -w workflow.ga -o tools.yaml
shed-tools install -g GALAXY -a API_KEY -t tools.yaml
workflow-install -g GALAXY -a API_KEY -w workflow.ga --publish-workflows