Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Editorial Board

This material is reviewed by our Editorial Board:

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Contributors

This material was contributed to by:

orcid logoNicola Soranzo avatar Nicola SoranzoEkaterina Polkh avatar Ekaterina Polkhorcid logoMateo Boudet avatar Mateo Boudetorcid logoMaria Doyle avatar Maria Doyleorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoRomane LIBOUBAN avatar Romane LIBOUBANJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoAnna Syme avatar Anna SymeSimon Bray avatar Simon Brayorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoFelicitas Kindel avatar Felicitas KindelNiall Beard avatar Niall Beardorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoSimon Gladman avatar Simon Gladmanorcid logoCristóbal Gallardo avatar Cristóbal GallardoDeepti Varshney avatar Deepti VarshneyAnton Nekrutenko avatar Anton NekrutenkoEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoDelphine Lariviere avatar Delphine LariviereTorsten Seemann avatar Torsten Seemannorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoBérénice Batut avatar Bérénice Batutorcid logoBjörn Grüning avatar Björn GrüningGildas Le Corguillé avatar Gildas Le Corguilléorcid logoPaul Zierep avatar Paul Ziereporcid logoHelena Rasche avatar Helena RascheTeresa Müller avatar Teresa MüllerKenji Fujihara avatar Kenji FujiharaWilliam Durand avatar William Durandorcid logoLaura Leroi avatar Laura Leroiorcid logoErwan Corre avatar Erwan CorreTwishi Gulati avatar Twishi GulatiBazante Sanders avatar Bazante SandersNathan Dunn avatar Nathan Dunn

Funding

These individuals or organisations provided funding support for the development of this resource