Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Community Resources

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Editorial Board

This material is reviewed by our Editorial Board:

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Contributors

This material was contributed to by:

orcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoHelena Rasche avatar Helena Rascheorcid logoAlexandre Cormier avatar Alexandre CormierAnton Nekrutenko avatar Anton NekrutenkoValentin Loux avatar Valentin LouxGildas Le Corguillé avatar Gildas Le Corguilléorcid logoNicola Soranzo avatar Nicola SoranzoBazante Sanders avatar Bazante SandersEsteban Perez-Wohlfeil avatar Esteban Perez-WohlfeilWilliam Durand avatar William DurandKenji Fujihara avatar Kenji FujiharaTorsten Seemann avatar Torsten Seemannorcid logoLaura Leroi avatar Laura Leroiorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoTeresa Müller avatar Teresa Müllerorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoSimon Gladman avatar Simon GladmanGuillaume Gautreau avatar Guillaume GautreauNiall Beard avatar Niall BeardSimon Bray avatar Simon Brayorcid logoAnna Syme avatar Anna Symeorcid logoPaul Zierep avatar Paul Ziereporcid logoErwan Corre avatar Erwan Correorcid logoBérénice Batut avatar Bérénice Batutorcid logoDeepti Varshney avatar Deepti VarshneyHélène Chiapello avatar Hélène ChiapelloEkaterina Polkh avatar Ekaterina Polkhorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoBjörn Grüning avatar Björn Grüningorcid logoFelicitas Kindel avatar Felicitas KindelTwishi Gulati avatar Twishi GulatiNathan Dunn avatar Nathan Dunnorcid logoMaria Doyle avatar Maria Doyleorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoMateo Boudet avatar Mateo BoudetJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoMiaomiao Zhou avatar Miaomiao Zhou

Funding

These individuals or organisations provided funding support for the development of this resource